General description

A cell-permeable steroidal alkaloid and cholesterol mimic that displays both teratogenic and antitumor activities. It disrupts cholesterol bio-synthesis and specifically antagonizes shh (Sonic Hedgehog) signaling pathway through direct interaction with Smo (smoothened), a distant relative of G-protein-coupled receptors. A valuable tool for studying the involvement of shh signaling in the development of various tumors. Tomatidine (Cat. No. 614350) can be used as a negative control. The solid form of this compound (Cat. No. 239803) is also available.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Berman, D.M., et al. 2003. Nature,425, 846.Thayer, S.P., et al. 2003. Nature,425, 851.Watkins, D.N., et al. 2003. Nature422, 313.Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA99, 14071.Berman, D.M., et al. 2002. Science297, 1559.Taipale, J., et al. 2000. Nature406, 1005.Kim, S.K., and Melton, D.A. 1998. Proc. Natl. Acad. Sci. USA95, 13036.Cooper, M.K., et al. 1998. Science280, 1603.Incardona, J.P., et al. 1998. Development125, 3553.

Packaging

5 mg in Glass bottle

Packaged under inert gas

Physical form

Supplied as a 10 mM (5 mg/1.22 mL) solution of Cyclopamine, V. californicum (Cat. No. 239803) in EtOH.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Flammable (J)